Journal: Biomedicines

Article Title: Stability of Blood DNA Methylation Across Two Timepoints in Three Cohorts

doi: 10.3390/biomedicines12112557

Figure Lengend Snippet: Example of top 5 probes by highest ICC from among most stable probes (with the lowest MAD, and highest ICC) with no SNP or gap identified by Gaphunter [ 27 ]. The p -value from the paired t-test is included. All the 172 probes were of design type I. Interestingly, WDR27 is thought to be an imprinted gene.

Article Snippet: The first dataset, by Almstrup et al. [ ] (E-MTAB-4187), included blood DNA methylation profiles obtained using the Illumina 450k in a group of children in pre- and post-pubertal period.

Techniques:

Journal: BMC Medicine

Article Title: The fetal origins of metabolic health: exploring the association between newborn biological age and metabolism hormones in childhood

doi: 10.1186/s12916-024-03629-z

Figure Lengend Snippet: Distribution on aging biomarkers and metabolism hormones. A Schematic of the study design. B–F Median and interquartiles for B TL, C mtDNAcn, D DNAmAge, E insulin, and F leptin. TL telomere length, mtDNAcn DNA copy number, DNAmAge DNA methylation age; *** P < 0.001

Article Snippet: This research involved 895 mother–child pairs from a birth cohort in China, with TL and mtDNAcn measured using quantitative real-time PCR, DNA methylation (DNAm) assessed using Infinium MethylationEPIC Beadchip, and DNAm age (DNAmAge) determined using Horvath’s epigenetic clock.

Techniques: DNA Methylation Assay

Journal: BMC Medicine

Article Title: The fetal origins of metabolic health: exploring the association between newborn biological age and metabolism hormones in childhood

doi: 10.1186/s12916-024-03629-z

Figure Lengend Snippet: Scatterplots and linear regression lines for aging biomarkers. Scatterplot and linear regression line for the comparison of A mtDNAcn (Ln-transformed) and TL (Ln-transformed), B DNAmAge (Ln-transformed) and TL (Ln-transformed), and C DNAmAge (Ln-transformed) and mtDNAcn (Ln-transformed). In scatter plots, orange lines indicate linear regression lines. Significance by two-tailed Pearson correlation; r, Pearson correlation coefficient. TL telomere length, mtDNAcn DNA copy number, DNAmAge DNA methylation age

Article Snippet: This research involved 895 mother–child pairs from a birth cohort in China, with TL and mtDNAcn measured using quantitative real-time PCR, DNA methylation (DNAm) assessed using Infinium MethylationEPIC Beadchip, and DNAm age (DNAmAge) determined using Horvath’s epigenetic clock.

Techniques: Comparison, Transformation Assay, Two Tailed Test, DNA Methylation Assay

Journal: BMC Medicine

Article Title: The fetal origins of metabolic health: exploring the association between newborn biological age and metabolism hormones in childhood

doi: 10.1186/s12916-024-03629-z

Figure Lengend Snippet: Dose–response relationships between newborn aging biomarkers and metabolism hormones in children analyzed by RCS. β (solid lines) and 95% CIs (shaded areas) of association of A TL (Ln-transformed) and insulin (Ln-transformed), B TL (Ln-transformed) and leptin (Ln-transformed), C mtDNAcn (Ln-transformed) and insulin (Ln-transformed), D mtDNAcn (Ln-transformed) and leptin (Ln-transformed), E DNAmAge (Ln-transformed) and insulin (Ln-transformed), and F DNAmAge (Ln-transformed) and leptin (Ln-transformed). β (95% CI) were adjusted for child sex (boys or girls), birth weight (continuous), season of delivery (warm or cold), gestational age at birth (continuous), passive smoking during pregnancy (yes or no), gestational weight gain (inadequate, recommended, excessive), children’s age, season of peripheral blood sampling (warm or cold), storage time of peripheral blood, maternal education status (≥ or < bachelor’s degree), paternal education status (≥ or < bachelor’s degree), and annual family income (≥ 100,000 yuan, 50,000–99,000 yuan, < 50,000 yuan). Horizontal dotted lines indicated effect values at the 10th percentile of the independent variable. RCS restricted cubic spline, Ln natural log-transformed, CI confidence interval, TL telomere length, mtDNAcn DNA copy number, DNAmAge DNA methylation age

Article Snippet: This research involved 895 mother–child pairs from a birth cohort in China, with TL and mtDNAcn measured using quantitative real-time PCR, DNA methylation (DNAm) assessed using Infinium MethylationEPIC Beadchip, and DNAm age (DNAmAge) determined using Horvath’s epigenetic clock.

Techniques: Transformation Assay, Sampling, DNA Methylation Assay

Journal: JAMA Pediatrics

Article Title: Longitudinal Changes in Epigenetic Age Acceleration Across Childhood and Adolescence

doi: 10.1001/jamapediatrics.2024.3669

Figure Lengend Snippet: List of Covariates

Article Snippet: Saliva samples were assayed for genome-wide DNA methylation profiling using Illumina EPIC or 450K methylation arrays for 874 and 1165 participants, respectively.

Techniques: Control, DNA Methylation Assay, Produced, Transformation Assay